Brown-to-white transition in subcutaneous fat: linking aging and disease

resistance increases with age, but molecular mechanisms remain unclear. Aging causes loss of subcutaneous white adipose tissue (sWAT) associated with a redistribution of fat from peripheral to central stores [1]. Interestingly, in older individuals the ratio of peripheral/central fat is a stronger determinant of insulin resistance than central adiposity alone [2]. This suggests sWAT is likely to be important, but age-dependent alterations specific to this depot remain poorly characterized; therefore, we recently investigated how murine sWAT changes as a function of age [3]. A unique feature of sWAT is the presence of highly oxidative brown adipocytes. However, our recent studies demonstrate an age-dependent disappearance of these brown adipocytes associated with the development of insulin resistance [3]. In murine sWAT, we observe a precipitous decline in expression of ucp1, the hallmark of a brown adipocyte, with advancing age (Figure 1). Remarkably, ucp1 levels decrease more than 800-fold between 3 and 12 months of age (Figure 1). Loss of sWAT 'browning' with age is further supported by reduced expression of other brown-indicator genes, including cidea, cox7a1, and ppara, as well as corresponding histological changes. What initiates this brown-to-white transition remains unclear, but adiposity is unlikely because animals that do not gain weight while aging display similar loss of ucp1. Older animals have reduced adrenergic receptor expression and increased expression of enzymes that degrade catecholamines, suggesting local adrenergic tone is a factor. Indeed, our unpublished findings reveal age-dependent alterations in a number of additional modulators of adrenergic signaling. Further, we show that administering an exogenous β-3 adrenergic receptor (β3AR) agonist effectively reverses the phenotype (Figure 1). Ongoing studies are directed towards a more detailed understanding how adrenergic signaling and other mechanisms promote these age-dependent changes in the phenotype of sWAT. Additional interesting questions generated by the findings include: 1. Do brown-like characteristics in human sWAT similarly decline with age? Cold-induced activity of Editorial brown adipose tissue found in humans is reduced with age [4]. Interestingly, human brown adipose tissue displays more similarities to murine 'beige' fat, or the brown adipocytes residing in sWAT, than classical brown fat [5]. Ucp1 is expressed in human sWAT [6], hence, evaluating age-dependent variation and its potential link with metabolic diseases will be important. 2. Is sWAT expression of ucp1 associated with diabetes status as humans age? Our studies reveal an inverse association between sWAT expression of ucp1 and hyperglycemia in older mice. It has been reported that …